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KMID : 0387420010120020173
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Korean Society of Oriental Neuropsychiatry
2001 Volume.12 No. 2 p.173 ~ p.183
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Studies on the anti-inflammatory action of Chilbokyeum extract in central nervous system
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Min Sang-Jun
Lee Sung-Ryull
Kang Hyung-Won
Lyu Yeoung-Su
Jeon Chang-Hwan
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Abstract
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Substance P can stimulate secretion of tumor necrosis $factor-\;{\alpha}\;(TNF-\;{\alpha}\;)$ from astrocytes stimulated with lipopolysaccharide (LPS). Here I report that Chilbogeum can modulate cytokines secretion from primary cultures of rat astrocytes. Chilbogeum $(10\;{\mu}g/ml)$ significantly inhibited the $TNF-\;{\alpha}$ secretion by astrocytes stimulated with LPS and Substance P. Interleukin-1 (IL-1) has been shown to elevate $TNF-\;{\alpha}$ secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. Treatment of Chilbogeum $(10,\;100\;{\mu}g/ml)$ to astrocytes stimulated with both LPS and Substance P decreased IL-1 secretion significantly. The secretion of $TNF-\;{\alpha}$ by LPS and Substance P in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Upon stimulation from various agents, these cells adopt a reactive phenotype, a morphological hallmark in Alzheimer’s disease (AD) pathology, during which they themselves may produce still more inflammatory cytokines. Chilbogeum $(10,\;100\;{\mu}g/ml)$ significantly inhibited the $TNF-\;{\alpha}$ secretion by CCF-STTG1 astrocytoma cells stimulated with $A\;{\beta}$ and IL-1. These results suggest that Chilbogeum may inhibit $TNF-\;{\alpha}$ secretion by inhibiting IL-1 secretion and that Chilbogeum has an antiinflammatory activity in AD brain.
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KEYWORD
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Tumor necrosis $factor-{\alpha}\, (TNF-\, {\alpha})$, astrocytes, Interleukin-1(IL-1), ${\beta}\, -amyloid\, (A\, {\beta})$, Chilbogeum, Substance P (SP), lipopolysaccharide (LPS)
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